Food Science I: Food Science and Nutrition, Properties of Food, Quality Evaluation, Effects of Cooking and Processing, Preservation, and Pigments and Additives
2. Quality evaluation: objective and subjective
Food quality is the sum of characteristics that decide acceptability: appearance (colour, size, shape, defects), texture and consistency, flavour (taste plus aroma), and nutritive and safety value. It is judged in two complementary ways. Objective evaluation uses instruments that give the same reading whoever operates them; subjective (sensory) evaluation uses a panel of people, because only people can say whether a food is liked.
| Objective method | What it measures |
|---|---|
| Colorimeter / Hunter L, a, b values | Lightness and colour coordinates |
| Refractometer | Total soluble solids in degrees Brix (juices, jams) |
| Viscometer; line-spread test | Flow and consistency of sauces, batters |
| Penetrometer; texture analyser | Firmness, hardness, chewiness |
| pH meter; titratable acidity | Acidity, which also governs safety and colour |
Sensory tests fall into three families. Difference tests ask whether a difference exists: the paired comparison, the duo-trio (which of two samples matches a reference) and the triangle test (three coded samples, two identical, pick the odd one; the chance of guessing right is one in three). Preference and acceptance tests ask how much a food is liked, most often on the nine-point hedonic scale running from "like extremely" to "dislike extremely". Descriptive tests, such as the flavour profile and quantitative descriptive analysis, use trained panellists to score the intensity of named attributes. Tests are run in booths under controlled light, with samples coded by random three-digit numbers, served in random order, with water to rinse the palate between samples.
3. Effects of cooking and processing, and the principles of preservation
Cooking improves digestibility, destroys many micro-organisms and anti-nutritional factors, and develops flavour, but it costs nutrients. Water-soluble vitamins leach into cooking water; vitamin C is destroyed by heat, air, alkali and copper; thiamine is heat-labile and is destroyed rapidly when baking soda is added to dal or vegetables; riboflavin is sensitive to light, which is why milk in clear bottles loses it; fat-soluble vitamins are comparatively stable, though carotene oxidises on long exposure. Losses are reduced by cooking in the minimum water, using the cooking water, covering the pan, cutting close to cooking time and avoiding soda.
- Milling and polishing remove the bran and germ of cereals and with them much of the thiamine, fibre and minerals.
- Parboiling rice drives water-soluble vitamins from the bran into the grain before milling, so parboiled rice keeps more thiamine.
- Germination raises vitamin C and some B vitamins and lowers phytate, improving iron and zinc availability.
- Fermentation (idli, dosa, dhokla, curd) raises some B vitamins, lowers anti-nutrients and improves digestibility.
- Blanching before freezing or drying inactivates enzymes that would otherwise cause off-flavours and browning in storage.
Every preservation method rests on one or more of three principles: preventing or delaying microbial decomposition (by keeping microbes out, removing them, hindering their growth or killing them), delaying self-decomposition by the food's own enzymes, and preventing damage by insects, rodents and physical causes. Low temperature slows growth (refrigeration at about 0 to 5 degrees Celsius, freezing at minus 18 degrees or below). High temperature kills: pasteurisation destroys pathogens in milk by the low-temperature long-time method (about 63 degrees Celsius for 30 minutes) or the high-temperature short-time method (about 72 degrees for 15 seconds), while sterilisation and canning aim at commercial sterility. Drying lowers water activity; most bacteria need a water activity above about 0.91, most moulds above about 0.80. Sugar and salt preserve by raising osmotic pressure, acids and fermentation by lowering pH, and chemical preservatives by inhibiting microbes. Irradiation is a cold process for sprout inhibition, insect disinfestation and pathogen reduction.
4. Food pigments
Colour is the first quality a consumer judges, and each natural pigment reacts in its own way to acid, alkali, heat and metals. Knowing these reactions explains why a vegetable turns dull or bright in a given recipe.
| Pigment | Found in | Behaviour |
|---|---|---|
| Chlorophyll (magnesium at the centre) | Green leafy vegetables, peas | Acid and long cooking replace magnesium, giving olive-brown pheophytin; alkali keeps it bright green but softens texture and destroys vitamins |
| Carotenoids | Carrot, mango, papaya, pumpkin, tomato (lycopene) | Fat-soluble, fairly heat-stable; beta-carotene is a vitamin A precursor; oxidise on drying and long storage |
| Anthocyanins | Jamun, red cabbage, black grapes, brinjal skin | Water-soluble; red in acid, violet to blue in alkali; turn dull with iron or tin |
| Anthoxanthins (flavones, flavonols) | Cauliflower, onion, potato, rice | White in acid, yellowish in alkali or hard water |
| Betalains | Beetroot | Water-soluble red-violet; leach readily |
| Myoglobin | Meat | Purple-red; bright red as oxymyoglobin; brown as metmyoglobin; pink as nitrosomyoglobin in cured meat |
5. Food additives
A food additive is a substance not normally eaten as a food by itself, added intentionally for a technological purpose in manufacture, processing, packing or storage. Additives are identified internationally by the International Numbering System (INS) of the Codex Alimentarius, are evaluated for an acceptable daily intake (ADI), and in India are permitted only for the foods and at the levels laid down in the food additive regulations framed under the Food Safety and Standards Act 2006.
| Class | Purpose | Examples |
|---|---|---|
| Preservatives | Inhibit microbes | Sodium benzoate, potassium metabisulphite, sorbic acid |
| Antioxidants | Delay oxidative rancidity | BHA, BHT, TBHQ, tocopherols, ascorbic acid |
| Emulsifiers and stabilisers | Keep oil and water mixed; give body | Lecithin, mono- and diglycerides, pectin, gums, CMC |
| Colours | Restore or give colour | Natural: turmeric, annatto, caramel; synthetic: tartrazine, sunset yellow FCF, carmoisine |
| Flavour enhancers and sweeteners | Intensify taste; replace sugar | Monosodium glutamate; aspartame, sucralose, saccharin |
| Acidity regulators, leavening agents, anti-caking agents | Control pH; aerate; keep powders free-flowing | Citric acid; baking powder; silicon dioxide |
Key takeaways
- Foods are colloids (emulsions, foams, gels, sols), and starch gelatinisation, dextrinisation, caramelisation, Maillard browning, protein coagulation and fat rancidity explain most cooking results.
- Objective evaluation uses instruments; sensory evaluation uses difference tests (triangle, duo-trio, paired), hedonic tests (the nine-point scale) and descriptive tests.
- Vitamin C and thiamine are the most vulnerable nutrients in cooking; soda destroys thiamine, light destroys riboflavin; parboiling, germination and fermentation raise nutritive value.
- Preservation works by keeping microbes out or killing them, slowing enzymes, lowering water activity or pH, or raising osmotic pressure.
- Chlorophyll dulls in acid, anthocyanins turn red in acid and blue in alkali, anthoxanthins yellow in alkali; additives are permitted, declared and limited, unlike adulterants.
Practice questions (10)
Attempt each one before opening the answer. Every explanation names the tempting wrong option as well as the right one, because that is where marks are lost.
Mayonnaise is best described as:
Show answer
Answer: C — An oil-in-water emulsion stabilised by lecithin
Mayonnaise disperses oil droplets in a watery phase of vinegar and egg yolk, and the lecithin of the yolk is the emulsifier. Butter is the classic water-in-oil emulsion; whipped cream is the foam.Browning of the crust of bread, which also lowers the availability of lysine, is due to:
Show answer
Answer: D — The Maillard reaction
The Maillard reaction joins reducing sugars with amino groups, so it browns the crust and ties up lysine. Caramelisation involves sugar alone; enzymatic browning needs polyphenol oxidase in cut produce; retrogradation is the re-crystallising of starch that stales bread.In a triangle test, 30 panellists each receive three coded samples, two of them identical. If the samples were truly indistinguishable, how many panellists would be expected to pick the odd sample correctly by chance alone? Type the number.
Numerical answer — type the value.
Show answer
Answer: 10
The probability of guessing the odd sample in a triangle test is 1/3, so the chance expectation is 30 × 1/3 = 10. Only a number of correct answers significantly above 10 shows a real perceptible difference.Which of the following reduce the loss of water-soluble vitamins when vegetables are cooked? Select all that apply.
Show answer
Answer: A — Cooking in the minimum amount of water and using that water; C — Covering the pan to shorten cooking time
Minimum water that is then eaten, and a covered pan that shortens heating, both cut losses. Baking soda destroys thiamine and vitamin C, and cutting small pieces early and soaking them multiplies the surface from which vitamins leach and oxidise.Why is potassium metabisulphite unsuitable for preserving jamun squash?
Show answer
Answer: A — The sulphur dioxide it releases bleaches the anthocyanin pigment
KMS releases sulphur dioxide, which decolourises red and purple anthocyanins, so coloured products such as jamun squash are preserved with sodium benzoate. KMS is a permitted preservative and works well in acidic light-coloured products.Assertion (A): Green vegetables cooked for a long time with a lid on, or with tamarind, turn olive-brown. Reason (R): Acid replaces the magnesium of chlorophyll with hydrogen, forming pheophytin. Choose the correct option.
Show answer
Answer: A — Both A and R are true, and R is the correct explanation of A
Both are true and R explains A. A lid traps the volatile plant acids released on cooking, and tamarind adds acid; in both cases magnesium is lost and olive-brown pheophytin forms. Cooking uncovered for a short time lets the acids escape.Which statements about anthocyanins and anthoxanthins are correct? Select all that apply.
Show answer
Answer: A — Anthocyanins are red in acid and turn violet to blue in alkali; D — Cauliflower cooked in hard, alkaline water turns yellowish because of its anthoxanthins
Anthocyanins are pH indicators, red in acid and blue in alkali, and anthoxanthins yellow in alkali. Anthocyanins are water-soluble and leach readily, and the vitamin A precursors are carotenoids such as beta-carotene, not anthoxanthins.Which instrument measures the total soluble solids of a fruit juice in degrees Brix?
Show answer
Answer: B — Refractometer
The refractometer reads the refractive index of a solution, which rises with dissolved solids and is calibrated in degrees Brix. The penetrometer measures firmness, the colorimeter colour and the viscometer flow.Which of the following are antioxidants used to delay rancidity in fats and fried snacks? Select all that apply.
Show answer
Answer: A — Tocopherols; B — BHA
Butylated hydroxyanisole and the tocopherols interrupt the free-radical chain of fat oxidation. Sodium benzoate is an antimicrobial preservative and carboxymethyl cellulose a thickener and stabiliser, so neither slows rancidity.Dried fruit with a water activity of 0.65 is unlikely to be spoiled by most bacteria mainly because:
Show answer
Answer: B — Most bacteria need a water activity above about 0.91 to grow
Water activity measures the water available to microbes; most bacteria need about 0.91 or more and most moulds about 0.80, so at 0.65 only some xerophilic moulds and osmophilic yeasts remain a risk. Drying does not sterilise and does not make fruit alkaline.